Manshan
Zhu^1,2, Dilin Liu^1,2, Wuge Liu^1,2, Dan Li^1,2,
Yilong Liao^1,2, Jinhua Li^1,2, Chongyun Fu^1,2,Fuhong
Fu^1,2, Huijun Huang^1,2, Xueqin Zeng^1,2,
Xiaozhi Ma^1,2 & Feng Wang^1,2

Scientific Reports

DOI:10.1038/s41598-017-10666-7

Abstract

To
dissect the genetic basis of yield formation in restorer line of hybrid rice,
we conducted QTL analysis for 6 yield traits including panicles per plant
(PPP), grains per panicle (GPP), grain yield per plant (GY), thousand-grain
weight (TGW), above-ground biomass (AGB), and harvest index (HI) using SNP
markers in a recombinant inbred lines (RILs) population derived from a cross
between a tropical japonica inbred Francis and an elite indica restorer
Guanghui 998 (R998). A total of 26 QTLs were detected using a high density
genetic map consisting of 3016 bin markers. Nineteen out of the 26 QTL alleles
from R998 had a beneficial effect on yield traits. Most of the QTLs were
co-located with previously reported rice QTLs. qAGB6 and qHI9, controlling AGB
and HI respectively, were detected as novel QTLs. Four QTLs for GY were
repeatedly detected across two years, with all the beneficial alleles from
R998. Notably, qGY8 explained over 20% of the yield variance in both years.
Moreover, qGY8 together with qTGW8 and qHI8 formed a QTL cluster. Markers
tightly linked with qGY8 were developed. Cloning of qGY8 will facilitate its
further exploitation in high-yield breeding.

附件：QTL mapping using an ultra-high-density SNP map reveals a major locus for grain yield in an elite rice restorer R998