Transcriptomic analysis reveals new insights into high-temperature-dependent glume-unclosing in an elite rice male sterile line
Chongyun
Fu1, 2, Feng Wang1, 2*, Wuge Liu1, 2, Dilin
Liu1, 2, Jinhua Li1, 2, Manshan Zhu1, 2,Yilong
Liao1, 2, Zhenrong Liu1, Huijun Huang1, 2,
Xueqin Zeng1, 2 and Xiaozhi Ma1, 2
Frontiers in Plant
Science
Abstract
Glume-unclosing
after anthesis is a widespread phenomenon in hybrid rice and also a maternal
hereditary trait. The character of Glume-unclosing in rice male sterile lines
also seriously influences germination rate and the commercial quality of hybrid
rice seeds. We validated that the type of glume-unclosing after anthesis in the
elite rice thermo-sensitive genic male sterile (TGMS) line RGD-7S was caused by
high temperature. Transcriptomic sequencing of rice panicles was performed to
explore the change of transcript profiles under four conditions: pre- and
post-anthesis under high temperature (HRGD0 and HRGD1), and pre- and
post-anthesis under low temperature (LRGD0 and LRGD1). We identified a total of
14,540 differentially expressed genes (DEGs) including some heat shock factors
(HSFs) across the four samples. We found that more genes were up-regulated than
down-regulated in the sample pair HRGD1vsHRGD0. These up-regulated genes were
significantly enriched in the three biological processes of carbohydrate
metabolism, response to water and cell wall macromolecular metabolism.
Simultaneously, we also found that the HSF geneOsHsfB1was specially
up-regulated in HRGD1vsHRGD0. However, the down-regulated DEGs in LRGD1vsLRGD0
were remarkably clustered in the biological process of carbohydrate metabolism.
This suggests that carbohydrate metabolism may play a key role in regulation of
glume-unclosing under high temperature in RGD-7S. We also analyzed the
expression pattern of genes enriched in carbohydrate metabolism and several HSF
genes under different conditions and provide new insights into the cause of
rice glume-unclosing.
